ATG7 antibody

Catalog No. ABIN7672901

This Mouse Monoclonal antibody specifically detects ATG7 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATG7 antibody (ABIN7672901)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 8H1

Product Details anti-ATG7 Antibody

Purpose: ATG7 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen: ATG7 autophagy related 7 homolog

Isotype: IgG2a

Application Details

Application Notes: WB: 1:1000-1:5000, IHC: 1:50-1:200, IF: 1:20-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background:

Synonyms: Ubiquitin-like modifier-activating enzyme ATG7|ATG12-activating enzyme E1 ATG7|Autophagy-related protein 7 (APG7-like, hAGP7)|Ubiquitin-activating enzyme E1-like protein|ATG7|APG7L

Background: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport(Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

Molecular Weight: 68-78 kDa

Gene ID: 10533

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy